Molecuar Characterization And Optimization Of Cellulase Producing Bacteria From Fruit & Vegetable Peel Wastes As A Substrate

Abstract

The aim of the present study was planned to isolate, identify, screening and molecular characterization of bacteria with high cellulase activity from fruit wastes and optimization of cellulase production. In the present study cellulase producing bacteria were isolated from fruit wastes on screening media containing Carboxy Methyl Cellulose (CMC). The organisms were identified using morphological and biochemical characterization. The two strains like Pseudomonas aeroginosa and Bacillus clausii were showed a sequence similarity upto 99% in 16S rRNA. Cellulase enzyme producing microorganism was isolated from fruit wastes and identified and molecularly characterised as Pseudomonas aeroginosa. The media was optimized for cellulase production and the isolate produces maximum cellulase at pH 9.1, temperature 40 ºC and 72 hrs of incubation time. Cellulase characterization shows that, cellulase has optimum activity at pH 5, temperature 45 ºC and 15 min of incubation with CMC as substrate. The Pseudomonas aeroginosa, showed the 78.03% yield of cellulase. Among bacteria, Pseudomonas aeroginosa, is the best cellulose producer among the four followed by Bacillus clausii, Cellulomonas sp, Escherichia coli, and Lactobacillus sp. Results shown that the bacterial isolates produced significant level of cellulase with cellulose degrading capability.